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Home :: Hemoglobin electrophoresis Hemoglobin ElectrophoresisHemoglobin (Hb) electrophoresis is probably the most useful laboratory method for separating and measuring normal and some abnormal Hb. Through electrophoresis, different types of Hb are separated to form a series of distinctly pigmented bands in a medium (cellulose acetate or starch gel). Results are then compared with those of a normal sample. Hb A, A2, S, C, and F are routinely checked, but the laboratory may change the medium or its pH to expand the range of this test. Purpose
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Reference valuesIn adults, Hb A accounts for 95% to 98% of all Hb; HbA2 1.5% to 3%; and HF, less than 2%. In neonates, Hb F normally accounts for half the total. Hb S and Hb C are normally absent. Interfering factors
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